Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Current Topics in Pharmacology   Volumes    Volume 1 
Selection of monoclonal antibodies to haptens for immnunoassays
Olivier Chappey, Pierre Sandouk, Jean-Michel Scherrmann
Pages: 43 - 55
Number of pages: 13
Current Topics in Pharmacology
Volume 1 

Copyright © 1992 Research Trends. All rights reserved

This review deals with the potency of monoclonal antibodies (MAbs) to haptens in immunoassays. Specificity and affinity of MAbs to haptens are the major determinants to be considered. Despite careful attention to both these requirements, polyspecific effects can occur leading to unforeseen cross-reactivity with compounds more or less related to the native hapten. This polyspecificity may be circumvented by the development of a large number of MAbs, as has been demonstrated for MAbs to cyclosporine. Affinity of MAbs to hapten is often lower than than of corresponding polyclonal antibodies (PAbs). Selection of appropriate specificity and affinity may depend on the analytical aim of the immunoassay. Pharmacokinetics requires MAbs with high specificity and affinity, while for therapeutic drug monitoring, toxicology or drug abuse detection a wider specificity and a lower affinity may be needed. If the strategy for selecting MAbs is carefully complied with, MAbs exhibit real advantages over classical PAbs to haptens because large amounts of worldwide standardized reagents can be prepared.
Buy this Article


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms