A human cell line selected for cisplatin resistance (CPR) was irradiated with ultraviolet (UV) and it showed cross resistance to UV. Applying modified CAT assay, we observed that CPR cells acquired enhanced host cell reactivation of transfected plasmid carrying UV damage. Gel mobility shift analyses indicate that two nuclear factors that recognize UV modified DNA (UVMD) were overexpressed in CPR cells. In addition, UVMD binding factors are independent from the factors that bind cisplatin modified DNA (CMD). The significance of the identified damage DNA binding factors, possibly DNA repair enzymes, is discussed.