In recent years, new technologies are emerging that allow a more detailed knowledge of different aspects of research in the life sciences. Classically, fluorescence activated cell sorting (FACS) has been of great help in the development of areas such as haematology or immunology. In this review, we will demonstrate that FACS can be used successfully in other fields, not only as a way to identify and separate different cellular types, but also to evaluate and quantify pharmacological responses, particularly in cells from the central nervous system.