Clostridium perfringens is a pathogen which produces many toxins and hydrolytic enzymes and is responsible for gangrene and gastrointestinal diseases. C. Perfringens toxins act on cell membrane by various mechanisms such as phospholipids cleavage and pore formation, leading to membrane damage and/or membrane permeability alteration, except ι-toxin which is active intracellularly and disrupts the actin cytoskeleton. α-toxin and ι-toxin display enzymatic activity (phospholipase C activity and actin ADP-ribosylation respectively). The other toxins are pore forming toxins or interact with membrane component by unknown mechanism. Hydrolytic enzymes contribute to tissue destruction in the gangrenous lesions.