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Trends in Chromatography   Volumes    Volume 1 
Chromatography of peptides in proteomics: accounts and perspectives
Renzo Bagnati, Roberta Pastorelli, Enrico Davoli
Pages: 63 - 70
Number of pages: 8
Trends in Chromatography
Volume 1 

Copyright © 2005 Research Trends. All rights reserved


Separation methods used in proteomics are generally based on 2-dimensional gels and liquid chromatography (LC). The use of liquid chromatography, after the electrospray ionization mass spectrometry development in the late 1980s, has been rapidly growing. 

State-of-the-art technologies in proteomics need both instrumental development for the mass spectrometer analyzer and for the chromatographic approach, e.g. high pressure chromatography or multiple LC separation dimensions as well as new capillary columns for micro and nano HPLC. New phases for better retention of polar compounds, new columns concepts, like the polymeric monolithic stationary phases as an alternative to the classical microparticulate sorbents, are emerging techniques that seem to be promising in this field.

A critical, more than an exhaustive review, is presented in this work for all new emerging techniques, to get a bird’s-eye view of this new, complex, analytical field.

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