Many biological assays are used to measure fluorescence intensity in order to ascertain the number of fluorophore labeled probes that bind selectively to the molecule of interest in the assay. As the bound groups of molecules emit light upon excitation, fluorophores can be easily detected through various fluorescence spectroscopy techniques, including emission acquisition scans. This research involved utilizing the properties of these fluorophores and investigating their use in novel ways such as utilizing their energy yields to power microsensors and switches. Of the solutes studied, Quinine, Rhodamine B, Popop, and Anthracene, Rhodamine B yielded the highest fluorescent intensity (3.98 x 106 counts per second (cps)), when dissolved in ethanol, as well as the highest power measurement, 10.46 picoAmps (pA). Cyclohexane, methanol, sulfuric acid, and distilled water did not dissolve Rhodamine B as well as ethanol. Research within the laboratory continues to involve the analysis of fluorophore properties and activity in select solvents to discover effective and resolute solvents that may be used for other novel methods related to sensing and micro-based chemicals for energy harvesting.
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