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Current Trends in Microbiology   Volumes    Volume 11 
Antimicrobial susceptibility testing performed via BD Phoenix™, broth microdilution, and disk diffusion for Pseudomonas aeruginosa
Jeanne B. Forrester, John A. Bosso, Barbara A. Santevecchi, Patrick Flume, Gloria E. Palmer-Long, Lisa L. Steed
Pages: 19 - 22
Number of pages: 4
Current Trends in Microbiology
Volume 11 

Copyright © 2017 Research Trends. All rights reserved

Antimicrobial susceptibility testing is often used to guide antibiotic selection for cystic fibrosis (CF) exacerbations; however, there is concern regarding the accuracy of automated systems, particularly for P. aeruginosa. The primary objective of this study was to evaluate the accuracy of the BD Phoenix™ Automated Microbiology System in determining susceptibility of P. aeruginosa. Clinical isolates form CF respiratory specimens were included. Susceptibility testing was performed via broth microdilution (BMD), disk diffusion (DD), and the BD Phoenix™. Minor, major, and very major errors were compared between the three methods. Forty-seven P. aeruginosa isolates were included; categorical agreement was 87.8% for the BD Phoenix™ and 87.9% for DD, compared to BMD. Both methods were found to have higher than acceptable error rates.
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