Barley doubled haploid (DH) lines derived from crossing common cultivars currently used in Europe and West Asia were tested for reaction to three important economic diseases, namely, common root rot (Cochliobolus sativus), leaf stripe (Pyrenophora graminea) and powdery mildew (Blumeria graminis) that cause significant annual yield losses. A broad spectrum of disease responses was observed for all pathogens ranging from highly susceptible to resistant which were consistent during the two field growth seasons. High levels of resistance were found in the new lines against powdery mildew (32.5%) and common root rot (25%), whereas overall resistance levels for leaf stripe were moderate (17.5%). Interestingly, two derived DH lines (B08-AS 34 and 35) were resistant to all three diseases that could be considered a valuable recourse for transferring of resistance genes in breeding programmes. However, the genetic diversity found among the promising DH lines with existence of remarkable levels of resistance, points to a high probability of finding novel sources of resistance in this collection that could be used against these three diseases in Mediterranean and similar environments.