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Current Topics in Peptide & Protein Research   Volumes    Volume 12 
Rapid separation of proteins from the aqueous media using the novel gelation protocol
Takashi Kadono, Yasuaki Fukuta, Kazuya Uezu, Hiroshi Morita, Kazuo Sakurai, Tomonori Kawano
Pages: 51 - 58
Number of pages: 8
Current Topics in Peptide & Protein Research
Volume 12 

Copyright © 2011 Research Trends. All rights reserved

Surfactants including those derived from the salts of fatty acids are often used for enhancing the solubility of proteins or conversely, for promotion of protein gelation in the aqueous media, thus applicable for separation of proteins from the biological samples. While the previously reported protein gelation protocols required the presence of highly concentrated proteins as the components of the gels, this report describes our recent attempts for capturing the proteins within the protein-independently formed surfactant-based gels which are rapidly formed after addition of surfactants and a metal-chelator to the solutions of proteins. Here, rapid isolation and removal of proteins from the liquid media using recently developed gel-forming formula with tetrasodium N,N-bis(carboxylatomethyl)-L-glutamate and sodium oleate are described. In the demonstrations, human hemoglobin, serum proteins, and mitochondrial proteins were successfully collected in the solid discs floating over the solution following centrifugation.
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