A simple and highly sensitive alternating current polarographic procedure is described for the determination of nilvadipine in pure form, in pharmaceutical preparations and in spiked human plasma. A well defined irreversible peak is obtained with Es of -0.96 V in 0.1 M HCl containing 20% (v/v) methanol. All the experimental parameters affecting the alternating current behavior of nilvadipine were studied and optimized. The relation between the concentration of nilvadipine and the summit current (is) was rectilinear over the ranges of 0.04-2.0 and 4-20 µg/ml with minimum detectability (S/N = 2) of 0.03 µg/ml (7.8 × 10-8 M). The summit current constant (n = 9) is 4.94 ± 0.05. The method was successfully applied for the determination of nilvadipine in commercial dosage forms. The results obtained were favorably compared with those obtained by a reference method. The method was further applied for the analysis of nilvadipine in spiked human plasma after simple extraction procedure with percentage recovery (n = 4) of 94.32 ± 1.46.
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