Non-albicans Candida yeast are regularly isolated and implicated in systemic mycoses. They can coexist with bacteria on different medical devices and create numerous poly-microbial infections with an impact on pathogenesis and patient health. The objective of our work is to study the multispecies infectivity of catheters collected at the University Hospital of Tlemcen (CHUT) and to take into consideration the association of non-albicans Candida/Enterobacteria and non-albicans Candida/Staphylococcus aureus. Samples were taken from catheters implanted in patients hospitalized at CHUT and the isolated microorganisms were subsequently identified. To define the different types of infectivity (contamination, colonization and infection), a microbial count was performed to determine the number of colony forming units. Statistical analysis was performed by khi square test. The present study shows a predominance of Candida tropicalis species, which are mostly co-isolated with enterobacteria. The distribution of microbial infectivity indicates that infection occupies the first place with a rate of nearly 63% of positive culture. No infections related to the combination (non-albicans Candida/S. aureus) were observed, whereas non-albicans Candida/Enterobacteria account for all infections of the devices sampled. The evaluation of the infectivity of the sampled medical devices showed a very high frequency of occurrence (about 90%) especially at the anesthesia-intensive care department and more frequently on the bladder catheters (93.5%). Medical devices at University Hospital of Tlemcen are a significant source of polymicrobial infection, specifically the intensive care unit, with a predominance of non-albicans Candida/Enterobacteria that is responsible for all catheter infections.