Infertility is among the diverse pathological conditions that beset the human society. Bisphenol A (BPA) is an endocrine-disrupting agent that was used as a monomer in the production of hard plastics. It can mimic the hormones of the body, and it can interfere with natural hormone production, absorption, transportation, action, function, and elimination. Oligomeric proanthocyanidins (OPC) is made up of bioflavonoids and polyphenols that act as free radical scavengers in the body. They can be found naturally in the bark, seeds, fruits and leaves of a vast range of plants in the plant kingdom including grapes, coffee and apple. The aim of the present research is to map the intergenerational effects of BPA and OPC intervention on the expression of Sult2a2, Sult2al1 and Sult2a1 genes. In this experimental study, 36 adult male Sprague Dawley rats weighing approximately 280 ± 20 grams aged 10 weeks old were randomly divided into 6 groups, namely negative control (NEC), BPA 200 mg/kg bwt (POC), low dose 10 μg/kg bwt OPC (OPC10), high dose 20 μg/kg bwt OPC (OPC20), BPA plus low dose OPC (BPA+OPC10) and BPA plus high dose OPC (BPA+OPC20) (n = 6 per group). Treatment and supplementation of BPA and OPC were conducted for 21 consecutive days before the male rats were mated with female rats at a ratio of 1:1. After the F1 generation were 10 weeks old, the same procedure and analyses were conducted. The expressions of Sult2a2, Sult2al1 and Sult2a1 in the blood were determined by oligonucleotide array-based comparative genomic hybridization (CGH). All data were analyzed by one-way analysis of variance (ANOVA) using SPSS software, version 21. The data were assessed at α = 0.05. There was significantly lower expression of Sult2a2, Sult2al1 and Sult2a1 genes in the POC group compared to the NEC group (p < 0.001) when compared both intragenerationally and intergenerationally. The number of gene deletion increased significantly from -0.85 in the P generation to -1.37 in the F1 generation. In contrast, the number of deleted genes in rats of the OPC10 group significantly decreased from -0.37 in the P generation to -0.14 in the F1 generation (p < 0.001). Similarly, rats that were treated with 200 mg/kg bwt BPA and then supplemented with 10 μg/kg bwt (BPA+OPC10) showed a significant decrease in terms of gene deletion, from -0.59 in the P generation to -0.46 in the F1 generation (p < 0.001). The remaining groups showed no significant difference (p > 0.05). OPC specifically at a dosage of 10 μg/kg bwt is able to alleviate the intergenerational detrimental effects of BPA on the expressions of Sult2a2, Sult2al1 and Sult2a1 genes.
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