We analysed, over an eight years period (2003-2011), the antimicrobial resistance profile of six bacterial pathogens of public health importance in humans namely Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis, Enterococcus faecium and Staphylococcus epidermidis, isolated from patients monitored in the Bone Marrow Transplant Center of Tunisia, to certain marker antibiotics. We observed the emergence of imipenem resistance in K. pneumonia and vancomycin resistance in E. faecium, an increase of high-level gentamicin resistance in E. faecalis and of third-generation cephalosporin resistance levels in E. coli, and a significant decrease in resistance to all antibiotic in P. aeruginosa with the exception of imipenem.