In an endeavor to find environmentally safer control measures, a project funded by the European Union-African, Caribbean and Pacific (EU/ACP) Initiative was implemented in the Southern African Development Community (SADC) region where white grubs cause severe damage and loss to sugarcane. In this study, the natural occurrence of entomopathogens was evaluated in diverse habitats in Mauritius and on TPC Ltd. in Tanzania. Insect pathogens were isolated by baiting soil samples with the standard bait insect, Galleria mellonella (Lepidoptera: Pyralidae). The sugar cane borers, Chilo sacchariphagus Bojer (Lepidoptera: Crambidae), Tetramoera schistaceana Snellen (Lepidoptera: Eucosmidae) and Eldana saccharina Walker (Lepidoptera: Pyralidae) were also tested as bait insects. This is the first report of the sugar cane borer, C. sacchariphagus successfully trapping entomopathogens from the soils. Field surveys and light trappings were conducted whereby scarab insects were collected and screened for the presence of pathogens. Samples of insect cadavers from Mauritius yielded 48 isolates of Metarhizium anisopliae and three of Beauveria spp. Other recorded fungal pathogens were Trichoderma harzanium Rifai (Hypocreales: Hypocreaceae), Clonostachys rosea (Link) Schroers (Hypocreales: Bionectriaceae) and Acremonium implicatum (Abbott) Gams (Hypocreales: Hypocreaceae). The two latter pathogens and the two Beauveria spp. were recorded for the first time on the sugar cane soft scale, Pulvinaria iceryi (Hemiptera: Coccidae) and the white grub species respectively under natural conditions locally. A total of 76 bacterial isolates were also isolated and cryopreserved. Regarding specimens from TPC sugar estate, ten consignments comprising of 453 infected insects were similarly shipped to ARC-SGI and these samples yielded 35 isolates of M. anisopliae and 22 of Beauveria spp. Ophiocordyceps sp., T. harzanium, C. rosea, the entomopathogenic nematode Steinernema sp. and bacterial isolates were also isolated. Pure cultures of the pathogens from the positive identifications from both countries were deposited in the South African National Collection of Fungi.