A mixed culture of Leifsonia shinshuensis and Rhodococcus zopfii was assessed on its ability to degrade carbamazepine (CBZ). The mixed culture was found to metabolise CBZ by utilizing two enzymes, oxygen oxidoreductase and aldehyde oxidase. Correlation analysis showed that the degraded CBZ was strongly correlated (R²≈0.9) with bacterial culture growth. However, only the concentrations of oxygen oxidoreductase is strongly correlated (R²≈0.9) with both degradation of CBZ and bacterial growth, whereas the aldehyde oxidase concentration was less strongly correlated (R²≈0.8) with both degradation of CBZ and bacterial growth. This suggests that the mixed culture adopted two different strategies simultaneously to metabolise CBZ to support growth and reduce toxicity.