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Current Topics in Toxicology   Volumes    Volume 18 
Abstract
Isolation and identification of cadmium-resistant bacteria that degrade crude oil
Efendy Chew, Wong Rui Rui, Ong Ghim Hock, Wong Kok Kee
Pages: 135 - 139
Number of pages: 5
Current Topics in Toxicology
Volume 18 

Copyright © 2022 Research Trends. All rights reserved

ABSTRACT
 
Three bacterial isolates were successfully screened and shown to have the ability to biodegrade crude oil and are resistant to cadmium at 0.1 mg/L. However, when the Cd concentration was increased from 0.1 mg/L to 1.0 mg/L, only one isolate, isolate C, was able grow. Gram staining results showed the isolate C to be Gram-negative. The DNA extracted from isolate C was amplified using a pair of 16s rDNA primers. The amplified DNA sequences were used to construct a phylogenetic tree. Analysis of the phylogenetic tree showed that 16s rRNA sequences from isolate C have the highest similarity to the 16s rRNA sequence of Pseudomonas aeruginosa.
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