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Current Topics in Peptide & Protein Research   Volumes    Volume 20 
Site-specific biotin-group conjugate of human Fas ligand extracellular domain: preparation and characterization of cell-death-inducing activity
Michiro Muraki, Kiyonori Hirota
Pages: 17 - 24
Number of pages: 8
Current Topics in Peptide & Protein Research
Volume 20 

Copyright © 2019 Research Trends. All rights reserved

Engineering of human Fas ligand extracellular domain (hFasLECD) protein can provide us with an advanced way for treatment or diagnosis of many serious diseases represented by cancers. In this study, we prepared a site-specific biotin group conjugate of hFasLECD and conducted the characterization of its cell-death-inducing activity. Precipitation of complexes using magnetic beads for affinity-based capturing revealed that the conjugate retained the original binding activity to both human Fas receptor extracellular domain and streptavidin. A significant sensitization effect on the cell-death-inducing activity against a colorectal cancer cell line, HT-29 cells, was observed for the pretreatment with human interferon-γ, accompanied by a synergistic effect of 5-fluorouracil. The findings suggested that the prepared hFasLECD conjugate would be applicable to the future development of medically useful devices to detect the counterpart receptors in biological specimens as well as novel cytotoxic agents against diseased cells.
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