Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Current Topics in Peptide & Protein Research   Volumes    Volume 21 
Vapreotide regulates neuronal ATPase activity
Andrea Induni, Alma Kemmling, Luciana Sielecki, María Graciela López Ordieres
Pages: 107 - 113
Number of pages: 7
Current Topics in Peptide & Protein Research
Volume 21 

Copyright © 2020 Research Trends. All rights reserved

Vapreotide is a somatostatin peptide analogue, which is commonly used for the treatment of oesophageal varicose veins in liver diseases. We study the effect of different concentrations of vapreotide on the ATPase activity of the cerebral and cerebellar membranes. Vapreotide 10-6 M produced the inhibition of Ca2+-ATPase activity, but not of other enzyme activities, suggesting a selective response according to the brain area and the preparation used as an enzyme source. Vapreotide at 10-9 to 10-6 M concentrations produced an inhibitory effect on Na+, K+-ATPase, resulting in an inhibitory concentration 50 (IC50) equal to 5.7 × 10-9 M and no changes in Mg2+-ATPase activity. Commercial enzymatic preparation of the pig cerebral cortex used as an enzyme source also produced an inhibitory enzyme effect. The vapreotide inhibitory effect was abolished by the addition of cSSTA, a somatostatin receptor antagonist. Besides, in crude membranes of cerebral cortex vapreotide produced [3H]-ouabain binding inhibition by direct interaction with the ouabain site (close to K+ site) in the enzyme Na+, K+-ATPase. The previous administration of the NOS inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME), increased [3H]-ouabain binding. In this experimental condition, vapreotide could not displace many ouabain molecules bound with high affinity to its site. All these experiments provided evidence that ATPase could be one of the target sites for the effects of vapreotide at the neuronal level.
View Full Article  


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms