Several lines of evidence suggest that vitamin D signaling may be implicated in the maintenance of the integrity of neuromuscular junction (NMJ). NMJ is a highly specialized chemical synapse formed between motoneuron and myofiber. As shown in vitamin D-deficient and VDR-null mice, defect in vitamin D signaling causes structural and functional alterations in NMJs. An accurate understanding of how vitamin D signaling contributes to the maintenance of NMJ homeostasis may provide valuable insights into vitamin D supplementation used to alleviate symptoms of geriatric syndrome including frailty, sarcopenia, and falls. This review will summarize the current knowledge regarding the role of vitamin D signaling in the maintenance of NMJ integrity.