Protein conformational diseases make the lives of millions miserable worldwide. Therefore, it is very important to search for molecules that inhibit protein misfolding. In our experiments, we investigated the anti-amyloidogenic effect of hydroxypropyl-β-cyclodextrin and trans-ferulic acid using α-chymotrypsin as a model protein at neutral pH in the presence of 55% ethanol. Both inhibitors proved to be effective in inhibiting aggregation and their effect was exerted in a concentration-dependent manner. Trans-ferulic acid has proven to be a very powerful anti-aggregation agent, as it almost completely eliminated the presence of aggregates in the sample at a concentration of 10 mM.
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