This study aims to show the effect of Doxorubicin on chicken embryos by injecting this drug into the eggs of chickens containing 10-day-old embryos. One hundred eggs were selected and divided evenly into four groups, each group containing 25 eggs. The first group was considered a control group (normal saline injection), and the 2^nd (T1 group), 3^rd (T2 group) and 4^th (T3 group) were injected with Doxorubicin according to the body surface area of the chicken egg at a dose of 20, 40 and 60 mg/m², respectively. In the current study embryos in Doxorubicin-injected groups showed morphological and histological changes that were directly proportional to the increased dose, including decrease in the body weight and length and histopathological changes in heart and liver tissue compared to control group. Also, a reduction in the hatching rate was observed for Doxorubicin-injected groups (T1, T2 and T3): 0% hatching rate compared to the control group that showed a 100% hatching rate. The doses of Doxorubicin used in this study caused a decrease in the weight and length of chicken embryos and led to the death of all fetuses, as well as caused tissue lesions in the heart and liver.