A microbial fuel cell is a well-known biological device that can convert chemical energy to electrical energy without the combustion process. This technology still provides a limit of operation owing to its structural cost like proton exchange membrane. In this study, the silica-modified ceramic separator was developed for use in the cellulose-fed MFC. The maximal current density and power density of 27.00 ± 0.10 A/m³ and 1.46 ± 0.05 W/m³ were obtained. This study would help in gaining new knowledge about using the silica-modified ceramic separator integrated with cellulose-fed MFC for electricity generation.