We know many protein misfolding diseases, from Alzheimer’s disease to type II diabetes, which are characterized by the presence of amyloid fibrils. The treatment of these diseases can be done by using molecules that prevent the formation of fibrils or break up already formed fibers. Polyphenolic compounds can play an important role in the prevention of amyloid-related diseases, as they are strong antioxidants and neuroprotective. Propolis is rich in polyphenols, and hence its ethanolic extract has an anti-amyloidogenic effect. In the present study, α-chymotrypsin was used as a model protein to investigate the anti-amyloidogenic properties of propolis extract and one of its bioactive components, trans-ferulic acid. The aggregate characterization was done by turbidity measurements, aggregation kinetics and Congo red binding assays. Our results showed that the propolis extract and trans-ferulic acid effectively inhibit the aggregation of α-chymotrypsin.