The cell damage caused by singlet molecular oxygen (¹O₂) has been extensively studied for a long time using different sources of ¹O₂. It was shown that ¹O₂ is very reactive towards many electron-rich organic functions and biological targets like lipids, proteins and DNA. Here is presented the synthesis of N,N’-di(2,3-dihidroxypropyl)-1,4-naphthalenedipropanamide (DHPN¹⁷O₂) and 9,10-diphenylanthracene (DPA¹⁷O₂) endoperoxides labeled with ¹⁷O₂. The ability of DHPN¹⁷O₂ to act as a source of [¹⁷O]-labeled singlet oxygen was checked by using water soluble disodium salt of anthracene 9,10-diyldiethyl disulfate (EAS) and linoleic acid as  chemical traps of ¹O₂.