Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

 Regional Subscription Agents/Distributors
Current Topics in Pharmacology   Volumes    Volume 4 
Bafilomycin A1, a selective inhibitor of V-ATPase: influence on pH regulation and effector functions of alveolar macrophages
Thomas A. Heming, Akhil Bidani
Pages: 163 - 172
Number of pages: 10
Current Topics in Pharmacology
Volume 4 

Copyright © 1998 Research Trends. All rights reserved

This article reviews the physiology of V-type H+- translocating ATPase (V-ATPase) in light of recent findings that the electrogenic H+ pump is expressed in the plasma membrane of alveolar macrophages (mø). The plasmalemmal pump plays a determinant role in regulation of the cytosolic pH (pHi) of alveolar mø. Selective inhibition of V-ATPase with the macrolide antibiotic bafilomycin A1 leads to collapse of the plasma membrane electrochemical H+ gradient and causes cytosolic acidosis. Treatment with bafilomycin A1 also impairs the antimicrobial and defense functions of the mø, depressing phagocytosis and intracellular killing of bacteria and reducing the stimulus-induced production of reactive oxygen species and tumor necrosis factor-α (a proinflammatory cytokine). Bafilomycin’s actions on these effector functions correlate with the drug-induced changes in pHi. The plasmalemmal V-ATPase probably serves to protect pH-sensitive cell functions from potentially deleterious effects of the acidic microenvironments common to sites of infection and from increases in metabolic acid production during mø activation.

Buy this Article


Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Search Products
Browse in Alphabetical Order : Journals
Browse by Subject Classification : Journals

Ordering Information Ordering Information
Downloadable forms Downloadable Forms