A cytogenetic monitoring study was conducted on a group of brickmaker workers (BMWs) in the Durango state of Mexico to investigate the genotoxic risk of occupational exposure to highly polluting fuels used for the production of brick. The compounds generated by this activity may cause genetic mutations and possible health consequences. The buccal micronucleus cytome assay was performed on 30 BMWs and compared to 30 control individuals. One buccal mucosa sample was collected from the 60 participants. The slides were stained and microscopically analyzed for micronucleated (MN) cells. Participants were of similar average age in the BMV and control groups (39.53 ± 13.89 vs 36.80 ± 12.65). Average exposure time of the BMWs was 13.36 ± 8.09 years. Subjects belonging to the exposure group did not use any protective measures during their work activities. The MN frequency in BMWs increased significantly in comparison to the control group (3.06 ± 0.25 vs. 1.50 ± 0.21, P<0.001). This study demonstrates that occupational exposure to smoke emissions that contain genotoxic compounds from brick production may result in a significant induction of DNA damage in buccal mucosa cells of brickmakers engaged in occupational exposure.