An analysis method to determine compositions of unknown cosmetic was established. The method is based on multi-instruments analysis technique coupled solvent extraction. The multi-instruments involve high-performance liquid chromatography (HPLC), X-ray diffraction (XRD) and Fourier transform infrared (FTIR). The cosmetic sample was separated and enriched into 4 fractions by solvent extraction in which water, heptanes, chloroform, tetrahydrofuran (THF) were used. The main compounds of the cosmetic were qualitative and quantitative analyzed. The compounds were defined as mercuric ammonium chloride, liquid paraffin, polyethylene glycol 200, 1,2-propanediol, glycerin, stearic acid, linoleic acid, linoleic acid methylester and α-linoleic acid ethyl ester.