Triacylglyceride lipases have critical roles in physiological mechanisms of insects like releasing of triglycerides from fat bodies for energy demand, digestion and reproduction. The current study revealed their role and properties in the extra-oral digestion of Andrallus spinidens by using specific substrate, pH and inhibitors as well as zymogram analysis. Results demonstrated that the enzyme had the highest activity at pH 9. At temperature 40°C, the enzyme showed the highest activity in addition to stability for 12 hours. Among used ions, Ca²⁺ and Mg²⁺ increased TAG-lipase activity but other used ions decreased it. Used specific inhibitors including PMSF, EGTA and EDTA statistically decreased the enzyme activity but TTHA had no effect on TAG-lipase activity.