Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Current Topics in Peptide & Protein Research   Volumes    Volume 8 
Abstract
Secreted antigens of Mycobacterium avium subsp. paratuberculosis: identity, characterisation, expression profiles and immunoreactivity
M. Lanigan, B. Shiell, G. Beddome, K. Bruce, J. Vaughan, P. Stiles, M. Tizard, D. Stewart, W. P. Michalski
Pages: 67 - 79
Number of pages: 13
Current Topics in Peptide & Protein Research
Volume 8 

Copyright © 2007 Research Trends. All rights reserved

ABSTRACT
 
We undertook to identify and express proteins of Mycobacterium avium subspecies paratuberculosis (MAP) for use in evaluating their potential as biomarkers for the presence of this organism in infected animals. Such proteins, produced synthetically, may also have potential for application as refined diagnostic antigens in enhancement of the γ-IFN assay. Using liquid-phase proteomics we fractionated the cell-free supernatants (CFS) of MAP grown in vitro and using automated N-terminal sequencing identified: Antigen D, SOD, MAP2609 and Ahp C. These four proteins, and along with Mp35K, Hsp65, Ahp D, P34 and PstS3, which were rationally-selected with reference to the Mycobacterium avium (MAV) and MAP genomes, were cloned and expressed in, then purified from E. coli. Several of these proteins were detected by antibodies present in the serum of animals experimentally-challenged with MAP; however, their utility as MAP biomarkers remains unclear.
Buy this Article


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms