Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Trends in Cancer Research   Volumes    Volume 8 
Abstract
Tumor promotion by TNF-α-inducing protein and its receptor nucleolin in human gastric carcinogenesis by Helicobacter pylori
Hirota Fujiki, Tatsuro Watanabe, Masami Suganuma
Pages: 97 - 112
Number of pages: 16
Trends in Cancer Research
Volume 8 

Copyright © 2012 Research Trends. All rights reserved

ABSTRACT
 
Helicobacter pylori is classified as the definitive carcinogen for stomach cancer in humans: It induces inflammation in cancer microenvironment of the stomach associated with induction of proinflammatory cytokines, such as tumor necrosis factor-α (TNF-α), an endogenous tumor promoter. The finding of tumor promoting activity by Helicobacter pylori membrane protein 1 (HP-MP1) made it possible to identify HP0596 gene from the complete genome sequence of H. pylori strain 26695. The HP0596 protein, consisting of 172 amino acids with 19 kDa, was named TNF-α-inducing protein (Tipα), a new carcinogenic factor of H. pylori. To study the mechanism of Tipα, we prepared His-tagged recombinant Tipα protein (rTipα), the His-tag attached to N-terminal methionine, and deletion mutant of Tipα (rdel-Tipα) lacking the N-terminal 6 amino acids including 2 cysteines. The rTipα protein formed an active homodimer, and rdel-Tipα was an inactive monomer. Crystal structure of rdel-Tipα, however, showed a heart-shaped homodimer without covalent bonds, indicating the importance of N-terminal region. Nucleolin was identified as a receptor of Tipα using the pull-down assay with anti-FLAG antibody in a mixture of mouse gastric cancer cell lysates and rTipα-FLAG. Specifically, the homodimer of rTipα directly binds to two-thirds of C-terminal nucleolin on cell surface. Binding inhibitors of nucleolin, such as AS1411 and lactoferrin, are discussed as tools of gastric cancer treatment in humans.
View Full Article  


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms