The GF14λ is one of the fifteen 14-3-3 proteins found in Arabidopsis. The 14-3-3 proteins are highly conserved in eukaryotic cells, and their functions are associated with many cellular activities that range from regulating enzyme activities to serving as signaling molecules. By using the green fluorescence protein (GFP) fusion approach, the GF14λ was localized to nucleus and cytoplasm. The cytoplasmic localization makes it possible for GF14l to interact with its ligand proteins in cytoplasm, whereas its nuclear localization suggests that GF14l might play other roles in plant cells. Reduced expression of GF14λ by using the antisense technique leads to reduced levels of  14-3-3 proteins in leaf cells and smaller plant size in Arabidopsis, suggesting that 14-3-3 proteins play important roles in Arabidopsis growth and development. Since GF14λ and other 14-3-3 proteins interact with many cellular proteins, the smaller plant size observed in GF14λ antisense plants may not be caused by the reduced expression of GF14λ alone, instead it is likely due to the disturbances of many cellular metabolisms in which 14-3-3 proteins participate. Interestingly, transgenic plants that overexpress GF14λ -GFP or GFP-GF14λ fusion proteins accumulate more biomass than wild-type and free GFP-expressing plants and these GFP-GF14λ fusion protein-expressing plants are also more drought tolerant, which is consistent with data obtained from transgenic cotton plants that overexpress GF14λ alone. It appears that overexpression of GF14λ is beneficial to plant growth and development in general.