A comparison of slit scanning densitometry, videodensitometry, flatbed scanner densitometry, and image analysis is presented for quantification of high performance thin layer chromatograms containing zones of the model dye rhodamine B. For the first three methods commercial instruments were used, i.e, CAMAG TLC Scanner 3, CAMAG Videodensitometer, and AR2i ChromImage flatbed scanner, respectively. Image analysis was performed using a digital camera and free ImageJ software. Information is provided on the construction and applications of the commercial instruments, and a review of recent publications on thin layer chromatography with image analysis is provided.