Hemodynamic effect of cladribine (CdA) was studied using a rat model after single dose. Male Sprague Dawley (SD) rats weighing between 300 - 400 g (n = 8 - 12 per group) were used. Each rat was acclimatized for one week before experiment, and then received a single dose of either 1 mg/kg CdA by intra-arterial injection (ia) or 2 mg/kg of the drug subcutaneously (sc). Hemodynamic recordings were collected via an indwelling catheter implanted into a left carotid artery continuously up to 3 hours after drug administration. A control group received saline and the same treatment as described for the CdA group. Differences between the treatment groups and control were assessed by ANOVA and paired student’s t-test, and considered significance when p<0.05. Systolic and diastolic blood pressure (SBP and DBP) were significantly increased shortly after CdA injection and continued to increase for 3 hours for the duration of the experiment (p<0.05). By the end of the experiment, SBP was increased significantly from 130 ± 13 to 152 ± 17 mmHg (+17%) and DBP 106 ± 8.1 to 124 ± 16 mmHg (+17%) after the 1 mg/kg ia injection (paired t-test p<0.05). Blood pressure was also increased after the 2 mg/kg sc dose, but the increase did not reach statistical significance. Further, heart rate (HR) was decreased significantly from 453 ± 54 to 422 ± 53 bpm (-7%) and 449 ± 70 to 386 ± 69 bpm (-14%) following both 1 mg/kg ia and 2 mg/kg sc injections (paired t-test p<0.05). In conclusion, CdA increased SBP and DBP, but decreased HR following a single dose in the rat model. The hemodynamic effects were greater after ia injection.
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