The protein content in a keratinized structure such as hair, nail, and feather, is approximately 80% of the total mass. We believe that human hair is a valuable source of protein for producing biocompatible materials, because it is easy to collect hair in large quantity at an individual level. A rapid and convenient protein extraction method was developed for quantifying human hair components, which we called the Shindai method. We developed novel procedures for preparing human hair protein films from the solution. The conversion ratios were 60-80%. We also prepared a flexible protein film, which remained intact with human skin for 40 h. Scanning electron microscopic observations showed that the films consisted of particles and filamentous structures. In fact, hair protein particles were conveniently prepared from the wet protein films. The average diameters of the particles were 0.5-10 mm, depending on the mechanical stimuli. The particles mainly consisted of a-keratin type I and II, and no significant degradation was observed during the procedure. Confocal microscopic observations indicated that the particles were irregular in shape and each particle consisted of smaller particle aggregates. These protein films and particles could be used in the fields of biomedical and cosmetic materials such as wound dressings, cosmetic films, blood analog fluids, and carriers for drug-delivery system as self-originating materials.