Water samples from boreholes were sampled from three different areas of Borikiri in Port Harcourt to determine some of their physico-chemical qualities and microbiological characteristics. Standard analytical techniques were employed in the investigation. The dominant bacteria in the boreholes were identified as Pseudomonas aeroginosa, Bacillus, Staphylococcus areus, Seratia mercens, Chromobacterium, E.coli, Klebsiella species. Distribution of microorganisms in the boreholes indicate that the counts for total aerobic bacteria ranged from 4.7 x 10² to 6.3 x 10⁵ cfu/ml while the coliform group had 0 to 9.0x 10² cfu/ml in the water samples. The presence of coliform in the samples posses a potential threat to the local inhabitants of Borikiri area. Analysis of physico-chemical parameters showed that pH, dissolved oxygen, chloride, iron, alkalinity and conductivity levels were in trace quantities below permissible limits, the accumulation of these elements over time in the ground water sources can predispose the life of the people to severe danger.