Biotin-binding immunoglobulins (B-Igs) in sera from patients with atopic dermatitis, a dermatitis different from atopic dermatitis and allergic disorders, were first detected in 1993. B-Igs were first detected by modified immunoelectrophoresis: the combination of ordinary immunoelectrophoresis and avidin-biotin technique. The modified immunoelectrophoresis was time-consuming, thus, a simplified method (immunofixation method) was developed. The modified immunoelectrophoresis and the immunofixation method are qualitative methods. We developed quantitative methods in the avidin- or F(ab’)2anti-Ig-coated multiwell format. A more effective method for quantifying B-Igs in sera was established. The clinical significance of B-Igs is discussed in the text.
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