Home | My Profile | Contact Us
Research Trends Products  |   order gateway  |   author gateway  |   editor gateway  
ID:
Password:
Register | Forgot Password

Author Resources
 Author Gateway
 Article submission guidelines

Editor Resources
 Editor/Referee Gateway

Agents/Distributors
 Regional Subscription Agents/Distributors
 
Current Topics in Biochemical Research   Volumes    Volume 15  Issue 2
Abstract
Application of random amplified polymorphic DNA-PCR technique in early diagnosis of bladder cancer using urine samples
Mohamed El-Far, Hassan Abol-Enein, Ashraf Zakaria, Mohammed El-Gedamy
Pages: 23 - 33
Number of pages: 11
Current Topics in Biochemical Research
Volume 15  Issue 2

Copyright © 2013 Research Trends. All rights reserved

ABSTRACT
 
The aim of this study is to examine the possible application of Random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) method, as a genetic test for identifying the significant genomic alterations in bladder cancer patients. DNAs were extracted from the exfoliated cells in the urine of 50 male bladder cancer patients, using the Phenol-Chloroform DNA extraction method, and the yielded DNAs were amplified with the BioA-09 random primer (5`-GGGTAACGCC-3`). The amplified PCR products from RAPD analysis were electrophoretically separated in agarose gels; banding profiles were visualized by ethidium bromide staining and demonstrated under ultraviolet light. The genomic alterations were clearly apparent in tumor RAPD-PCR patterns, by the loss of normal bands and the appearance of new tumor-related bands, as compared to the controls. The correlation between the genomic alteration and the histopathological subtypes was assessed using the X2 test. In addition, bands with molecular sizes 500 (p < 0.001) and 1300 (p = 0.033) bp were used as molecular markers for transitional cell carcinoma, while squamous cell carcinoma type had two diagnostic bands with molecular sizes 1800 (p < 0.001) and 1400 (p < 0.021) bps, respectively. Our study suggests the potential of RAPD-PCR method to produce diagnostic markers for analysing the genomic instabilities in bladder tumors.
View Full Article  


 
search


E-Commerce
Buy this article
Buy this volume
Subscribe to this title
Shopping Cart

Quick Links
Login
Search Products
Browse in Alphabetical Order : Journals
Series/Books
Browse by Subject Classification : Journals
Series/Books

Miscellaneous
Ordering Information Ordering Information
Downloadable forms Downloadable Forms