High-intensity 260nm laser pulses induce base-sequence specific lesions in DNA through excitation involving the absorption of more than one photon and subsequent generation of purine and pyrimidine radicals. Although many details of the physical and chemical mechanisms remain still unknown, it appears that laser photochemistry could be a very interesting tool for studying DNA-ligand (proteins, drug….) interactions and for gaining insights in local DNA conformational features. The most important advantages over known methods, including conventional low-intensity photochemistry, is the rapidity of the process leading to photolesion formation that only requires a single short laser pulse. This would allow conformational dynamic studies to be carried out either in-situ or even within cells. Since laser photochemistry of DNA has been extensively reviewed during the last decade, our objective here is to critically survey some of the available information in light of its possible use for studies on DNA conformation and DNA-protein interaction. However, it should be kept in mind that further investigations are necessary for a better interpretation of the available data and to ensure further methodology development.