The authors investigated the effect of light quality on the content of chlorophylls, carotenoids and phytochrome in 14 species of bryophytes. The total content of chlorophylls and carotenoids was calculated from the formulae and the presence of the particular carotenoid was identified by column, thin-layer and high-performance liquid chromatography. The content of the phytochrome proteins was determined by electrophorese with polyacrylamine gel. The content of the four types of rays (red, yellow, green, blue) is different in particular months, in the sunny and cloudy days, in the different time of the day and in the shade of the particular trees. The gametophytes of the 14 species of bryophytes examined chlorophyll a and b and 25 carotenoids among which β-carotene, β-cryptoxanthin, lutein, and lutein epoxide occurred in the all species. All the species of bryophytes examined showed a relatively high level of chlorophyll b as compared to chlorophyll a. In late spring and early summer some species of bryophytes showed increased content of photosynthesizing pigments in their gametophytes, in all colour of light, from white to blue. In early spring, and late autumn in moderate climate, when light and humidity parameters improve (lack of vegetation and red rays are not absorbed; precipitation), in many species of bryophytes the biosynthesis of photosynthesizing pigments is intensified by red rays, phytochromes are activated and biomass of bryophytes increases.