Campanula rotundifolia  calli produced polyacetylene glucoside lobetyolin (over 0.06% dry weight, on Woody Plant medium with NAA-BA) and phenyl glucoside coniferin (0.62% dry weight, on 1/2 Murashige-Skoog medium with NAA- BA). The maximum concentration (0.28% dry weight) of lobetyolin in C. rotundifolia adventitious roots was almost double that of the root portion of the in vitro shoot cultures. C. rotundifolia hairy roots transformed by the infection with Agrobacterium rhizogenes ATCC 15834 produced 0.76% dry weight of lobetyolin and 0.32% dry weight of coniferin in the cultures. Regenerants from the transformed roots produced high concentration of lobetyolin and coniferin compared to those of the regenerants from adventitious roots and non-transformed in vitro shoot cultures. The regenerants from transformed roots showed typical hairy root syndrome such as dwarf plantlet, wrinkled leaf, root proliferation and many leaves formation and also exhibited strong tolerance for drought stress.