The synthesis of 13C labeled dihydroxy acetone (DHA) from 13C labeled methanol was accomplished in 4 synthetic Steps. The reactions have been scaled to provide gram quantities labeled DHA. The syntheses of site specifically labeled 2`-β-deoxyadenosine and 2`-β-deoxyguanosine have been achieved using a unified approach to labeling multiple purine sites. The synthesis of both 2`-β-[1,3-15N2]deoxyguanosine and 2`-β-[2,4,8-13C3-amino,3,9-15N4]deoxyguanosine demonstrates the feasibility of this unified route to labeled purine based nucleic acids.
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