The fatty acid composition of Biomphalaria glabrata snails maintained at 17 (cold), 24 (ambient), and 31 °C (warm) was determined by using gas chromatography-mass spectrometry (GC-MS). Snails were necropsied following two weeks of maintenance at the above temperatures and the whole snail bodies were pooled to provide samples that were later analyzed. Palmitic (16:0), eicosanoic (20:1), stearic (18:0), α-linoleic (18:3 n-3), and linoleic (18:2 n-6) acids comprised 67% of the total fatty acids in the snails maintained at 31 ºC. α-Linoleic (18:3 n-3), palmitic (16:0), eicosanoic (20:1), stearic (18:0), and linoleic acids (18:2 n-6) comprised 66% of the fatty acids of the snails maintained at 24 ºC. Eicosanoic (20:1), stearic (18:0), linoleic (18:2 n-6), oleic (18:1), and α-linoleic acid (18:3 n-3) comprised 63% of the fatty acids of the snails maintained at 17 ºC. No significant differences (ANOVA) were found in any of the individual fatty acids as a function of maintaining the snails at the three different temperatures. However, there were significant differences in the total combined unsaturated versus combined saturated fatty acid content among the snails maintained at the three different temperatures.